AuthentiKine™ Human IL-28A ELISA Kit, 96 tests

KE00237 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-28A ELISA kit is to be used to detect and quantify protein levels of endogenous IL-28A. The assay recognizes human IL-28A and IL-28B. An antibody specific for IL-28A has been pre-coated onto the microwells. The IL-28A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-28A is added to detect the captured IL-28A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Storage conditions:

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