AuthentiKine™ Human LIF ELISA Kit, 96 tests

KE00208 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The LIF ELISA kit is to be used to detect and quantify protein levels of endogenous LIF. The assay recognizes human LIF. An antibody specific for LIF has been pre-coated onto the microwells. The LIF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for LIF is added to detect the captured LIF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

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