Human GM-CSF ELISA Kit, 96 tests

KE00003 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GM-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous GM-CSF. The assay recognizes human GM-CSF. An antibody specific for GM-CSF has been pre-coated onto the microwells. The GM-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for GM-CSF is added to detect the captured GM-CSF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Storage conditions:

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