Human KIM-1/HAVCR1 ELISA Kit, 96 tests

KE00136 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The KIM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous KIM-1. The assay recognizes human KIM-1. An antibody specific for KIM-1 has been pre-coated onto the microwells. The KIM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for KIM-1 is added to detect the captured KIM-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Storage conditions:

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