Human Vitamin D BP ELISA Kit, 96 tests

KE00272 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Vitamin D BP ELISA kit is to be used to detect and quantify protein levels of endogenous human Vitamin D BP. The assay recognizes human Vitamin D BP. An antibody specific for human Vitamin D BP has been pre-coated onto the microwells. The human Vitamin D BP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Vitamin D BP is added to detect the captured human Vitamin D BP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

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