Human ZAG/AZGP1 ELISA Kit, 96 tests

KE00230 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The AZGP1 ELISA kit is to be used to detect and quantify protein levels of endogenous AZGP1. The assay recognizes human AZGP1. An antibody specific for AZGP1 has been pre-coated onto the microwells. The AZGP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human AZGP1 is added to detect the captured human AZGP1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Storage conditions:

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